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spark multimode microplate reader  (Tecan Systems)


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    Structured Review

    Tecan Systems spark multimode microplate reader
    Spark Multimode Microplate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 17087 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multimode+microplate+reader/Spark/pmc12997294-76-5-9
    Average 99 stars, based on 17087 article reviews
    spark multimode microplate reader - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Engineering UvsY solubility through local hydrophobic cluster disruption enables enhanced recombination mediator activity.
    Article Snippet: The recombination mediator UvsY plays a critical role in recombinase polymerase amplification but is intrinsically limited by poor solubility and aggregation at high protein concentrations.. Here, we present a systematic strategy to improve UvsY solubility and functional performance by integrating consensus mutagenesis with virtual saturation mutagenesis.. A single substitution, F73P, was identified as a core solubility-enhancing mutation that enables UvsY to remain soluble at concentrations exceeding 137.5 μg/μL and during long-term storage.

    Fluorescence:

    Article Title: Engineering UvsY solubility through local hydrophobic cluster disruption enables enhanced recombination mediator activity.
    Article Snippet: The recombination mediator UvsY plays a critical role in recombinase polymerase amplification but is intrinsically limited by poor solubility and aggregation at high protein concentrations.. Here, we present a systematic strategy to improve UvsY solubility and functional performance by integrating consensus mutagenesis with virtual saturation mutagenesis.. A single substitution, F73P, was identified as a core solubility-enhancing mutation that enables UvsY to remain soluble at concentrations exceeding 137.5 μg/μL and during long-term storage.

    Article Title: Fasting Enhances Cardiomyocyte Hypoxia Tolerance by Regulating Ca 2+ Transport at Mitochondria-Endoplasmic Reticulum Contact Sites.
    Article Snippet: For ATP content determination, samples were processed according to the manufacturer’s instructions of the ATP Assay Kit (E-BC-F002, Elabscience Biotechnology Co., Ltd., Wuhan, China). .. The fluorescence value of the samples was measured using a multimode microplate reader (SPARK, TECAN, Männedorf, Switzerland), and ATP content in samples was calculated based on the standard curve fitting. .. For apoptosis analysis, cells were stained with the Annexin V-FITC/PI Apoptosis Detection Kit (556547, BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer’s protocol.

    Membrane:

    Article Title: Two Bile Salt Hydrolase Homologues Drive Bile Resistance in Lactobacillus johnsonii ATCC 43121.
    Article Snippet: The role of bile salt hydrolase (BSH) in Lactobacillus johnsonii’s intestinal adaptability remains unclear.. Here, we elucidate the functions and mechanisms of three BSH homologues (BSHA, BSHB, and BSHC) in mediating bile resistance in Lb. johnsonii ATCC 43121, using bsh knockout and complementation mutants and functional assays under simulated human small intestinal pH conditions.. Functional tests showed BSHA and BSHC significantly enhanced the strain’s resistance to conjugated bile acids, whereas BSHB was ineffective due to low enzymatic activity.

    Luciferase:

    Article Title: Delivery of Circular RNAs into Splenic Immune Cells via Intravenous Administration of Polyaspartamide Derivative Polyplexes.
    Article Snippet: .. The photoluminescence intensity of the cell lysate was measured using a Luciferase Assay System (Promega) in a multimode microplate reader (Spark, Tecan Trading AG, Switzerland). ..



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